Overview

  • Product name

    Goat Anti-Chicken IgY H&L (FITC)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, IHC-Frmore details
  • Immunogen

    Chicken IgY

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.2% BSA

    Isotonic 0.9%, w/v)
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Ab46969 is specific to chicken IgY and will not cross react with mammalian IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-Fr
  • Application notes
    ICC/IF: 1/250.
    IHC-Fr: Use at an assay dependent dilution (PMID 18583367).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Images

    • ab4674 staining GFAP in human brain cells by Flow Cytometry. Cells were cultured in stem cell media RHB-A and collected using 0.05% Trypsin. The sample was incubated with the primary antibody (1/100 in PBS + 10% FCS) for 20 minutes at 25°C. A FITC-conjugated Goat anti-chicken IgY (ab46969) (1/100) was used as the secondary antibody.

       

      See Abreview

    References

    This product has been referenced in:

    • Nugent AA  et al. Mutant a2-chimaerin signals via bidirectional ephrin pathways in Duane retraction syndrome. J Clin Invest 127:1664-1682 (2017). Read more (PubMed: 28346224) »
    • Okodo M  et al. Cytological Features Associated with Ureaplasma Urealyticum in Pap Cervical Smear Asian Pac J Cancer Prev 18:2239-2242 (2017). Read more (PubMed: 28843262) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Answer

    DISCOUNT CODE for ab4674 Anti-GFAP antibody - Astrocyte Marker: XXXXX
    DISCOUNT CODE for ab46969 Goat anti-Chicken IgY H&L (FITC) secondary antibody: YYYYYYY


    Expiration date: ZZZZZZZ

    I am very pleased to hear your customer would like to test bothab4674 andab46969 in Flow Cytometry. The code will give them 1 free primary antibody and 1 secondary antibody before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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