Overview

  • Product name

    Goat Anti-Chicken IgY H&L (FITC)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
  • Clonality

    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Images

  • Ab6873 was used at dilution 1/500 with the primary antibody ab39376 in ICC/IF. See the review on ab39376.
  • Ab6873 was used at dilution 1/500 with the primary antibody ab13991 in IHC-Fr. See the review on ab13991.

References

This product has been referenced in:

  • Wu H  et al. Ubiquitination Is Essential for Avibirnavirus Replication by Supporting VP1 Polymerase Activity. J Virol 93:N/A (2019). Read more (PubMed: 30429342) »
  • Munera D  et al. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun 5:3080 (2014). Read more (PubMed: 24445323) »
See all 4 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Antibody was diluted 1/1000 in PBS with 1% goat serum and incubated 1h at RT

Abcam user community

Verified customer

Submitted Jul 29 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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