Key features and details
- Goat Anti-Chicken IgY H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
Our Abpromise guarantee covers the use of ab6873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6873 has been referenced in 4 publications.
- Wu H et al. Ubiquitination Is Essential for Avibirnavirus Replication by Supporting VP1 Polymerase Activity. J Virol 93:N/A (2019). PubMed: 30429342
- Munera D et al. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun 5:3080 (2014). PubMed: 24445323
- Millet YA et al. Insights into Vibrio cholerae intestinal colonization from monitoring fluorescently labeled bacteria. PLoS Pathog 10:e1004405 (2014). IF ; Human . PubMed: 25275396
- Sully K et al. The mTOR inhibitor rapamycin opposes carcinogenic changes to epidermal Akt1/PKBa isoform signaling. Oncogene : (2012). PubMed: 22890326