Goat Anti-Chicken IgY H&L (FITC) preadsorbed (ab7114)

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (FITC) preadsorbed
    See all IgY secondary antibodies

  • Host species

    Goat

  • Target species

    Chicken

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details

  • Minimal
    cross-reactivity


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • Immunogen

    Full length native chicken IgY (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG

  • Clonality

    Polyclonal

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:

  • Kokona D  et al. Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation. J Neuroinflammation 15:340 (2018). Read more (PubMed: 30541565) »
  • Ebneter A  et al. Microglia Activation and Recruitment of Circulating Macrophages During Ischemic Experimental Branch Retinal Vein Occlusion. Invest Ophthalmol Vis Sci 58:944-953 (2017). Read more (PubMed: 28170538) »
See all 6 Publications for this product

Publishing research using ab7114? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I have your chicken GFAP primary antibody (ab4674). What fluorescent-labled secondary do you recommend? I am looking for any color other than red. I have ab7114--would this work? If not, then why?

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Answer

Thank you for your email. At the bottom of the online datasheet for ab4674 there is a list of compatible secondary antibodies that can be used with ab4674. Ab7114 -Goat polyclonal to Chicken IgY H and L (FITC) - is a suitable secondary to use with ab4674. And since this secondary is conjugated to FITC (Fluorescein) the color emitted will be green. If you have any additional questions, please contact us again.

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