Product nameGoat Anti-Chicken IgY H&L (FITC) preadsorbed
See all IgY secondary antibodies
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details
Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native chicken IgY (purified).
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
Our Abpromise guarantee covers the use of ab7114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
|ELISA||1/10000 - 1/50000.|
This product has been referenced in:
- Kokona D et al. Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation. J Neuroinflammation 15:340 (2018). Read more (PubMed: 30541565) »
- Ebneter A et al. Microglia Activation and Recruitment of Circulating Macrophages During Ischemic Experimental Branch Retinal Vein Occlusion. Invest Ophthalmol Vis Sci 58:944-953 (2017). Read more (PubMed: 28170538) »