Goat Anti-Chicken IgY H&L (FITC) preadsorbed (ab7114)
Key features and details
- Goat Anti-Chicken IgY H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISA
Overview
-
Product name
Goat Anti-Chicken IgY H&L (FITC) preadsorbed
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details -
Immunogen
Full length native chicken IgY (purified).
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG -
Clonality
Polyclonal -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent dilution.
|
|
| IHC-Fr |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent dilution. |
|
IHC-Fr
Use at an assay dependent dilution. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (6)
ab7114 has been referenced in 6 publications.
- Kokona D et al. Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation. J Neuroinflammation 15:340 (2018). PubMed: 30541565
- Ebneter A et al. Microglia Activation and Recruitment of Circulating Macrophages During Ischemic Experimental Branch Retinal Vein Occlusion. Invest Ophthalmol Vis Sci 58:944-953 (2017). PubMed: 28170538
- Lucchetta EM & Ohlstein B Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine. Cell Stem Cell 20:609-620.e6 (2017). PubMed: 28343984
- Kokona D et al. Noninvasive Quantification of Retinal Microglia Using Widefield Autofluorescence Imaging. Invest Ophthalmol Vis Sci 58:2160-2165 (2017). PubMed: 28395300
- Liu YP et al. Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab 29:780-91 (2009). IHC-FoFr . PubMed: 19209181
- Ofosu FA et al. Coordinate Activation of Human Platelet Protease-activated Receptor-1 and -4 in Response to Subnanomolar {alpha}-Thrombin. J Biol Chem 283:26886-93 (2008). Flow Cyt . PubMed: 18682394
