For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    goat-chicken-igy-hl-fitc-preadsorbed-ab7114.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Secondary antibodies anti-Chicken IgY Fluorophore FITC
Share by email

Goat Anti-Chicken IgY H&L (FITC) preadsorbed (ab7114)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Goat Anti-Chicken IgY H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISA

You may also be interested in

Blocking
Product image
Normal Goat Serum (ab7481)

View more associated products

Overview

  • Product name

    Goat Anti-Chicken IgY H&L (FITC) preadsorbed
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native chicken IgY (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
  • Clonality

    Polyclonal
  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • FITC

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7114 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Flow Cyt
    Use at an assay dependent dilution.
    ELISA
    1/10000 - 1/50000.
    Notes
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Flow Cyt
    Use at an assay dependent dilution.
    ELISA
    1/10000 - 1/50000.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab7114? Please let us know so that we can cite the reference in this datasheet.

    ab7114 has been referenced in 6 publications.

    • Kokona D  et al. Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation. J Neuroinflammation 15:340 (2018). PubMed: 30541565
    • Ebneter A  et al. Microglia Activation and Recruitment of Circulating Macrophages During Ischemic Experimental Branch Retinal Vein Occlusion. Invest Ophthalmol Vis Sci 58:944-953 (2017). PubMed: 28170538
    • Lucchetta EM & Ohlstein B Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine. Cell Stem Cell 20:609-620.e6 (2017). PubMed: 28343984
    • Kokona D  et al. Noninvasive Quantification of Retinal Microglia Using Widefield Autofluorescence Imaging. Invest Ophthalmol Vis Sci 58:2160-2165 (2017). PubMed: 28395300
    • Liu YP  et al. Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab 29:780-91 (2009). IHC-FoFr . PubMed: 19209181
    • Ofosu FA  et al. Coordinate Activation of Human Platelet Protease-activated Receptor-1 and -4 in Response to Subnanomolar {alpha}-Thrombin. J Biol Chem 283:26886-93 (2008). Flow Cyt . PubMed: 18682394

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I have your chicken GFAP primary antibody (ab4674). What fluorescent-labled secondary do you recommend? I am looking for any color other than red. I have ab7114--would this work? If not, then why?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2005

    Answer

    Thank you for your email. At the bottom of the online datasheet for ab4674 there is a list of compatible secondary antibodies that can be used with ab4674. Ab7114 -Goat polyclonal to Chicken IgY H and L (FITC) - is a suitable secondary to use with ab4674. And since this secondary is conjugated to FITC (Fluorescein) the color emitted will be green. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.