Goat Anti-Chicken IgY H&L (FITC) preadsorbed (ab7114)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product name
    Goat Anti-Chicken IgY H&L (FITC) preadsorbed
    See all IgY secondary antibodies
  • Host species
    Goat
  • Target species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native chicken IgY (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Goat IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Liu YP  et al. Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats. J Cereb Blood Flow Metab 29:780-91 (2009). IHC-FoFr . Read more (PubMed: 19209181) »
  • Ofosu FA  et al. Coordinate Activation of Human Platelet Protease-activated Receptor-1 and -4 in Response to Subnanomolar {alpha}-Thrombin. J Biol Chem 283:26886-93 (2008). Flow Cyt . Read more (PubMed: 18682394) »

See all 2 Publications for this product

Publishing research using ab7114? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

I have your chicken GFAP primary antibody (ab4674). What fluorescent-labled secondary do you recommend? I am looking for any color other than red. I have ab7114--would this work? If not, then why?

Thank you for your email. At the bottom of the online datasheet for ab4674 there is a list of compatible secondary antibodies that can be used with ab4674. Ab7114 -Goat polyclonal to Chicken IgY H and L (FITC) - is a suitable secondary to use with ab46...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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