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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Chicken IgY H&L (HRP) (ab205721)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab205721)

Key features and details

  • Goat Anti-Chicken IgY H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, IP, ELISA, IHC-P

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Specificity

    The antibody used for conjugation reacts with chicken immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182019): Human IgG, mouse IgG, rat IgG and rabbit IgG, less than 2%.
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Enzyme
    • HRP

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab205721 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/2000 - 1/20000.
    IP Use at an assay dependent concentration.
    ELISA Use at an assay dependent concentration.
    IHC-P 1/1000 - 1/10000.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab205721)

      IHC image of alpha tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab89984 at 5ug/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature.

      An HRP-conjugated secondary (Ab205721, 1/2000 dilution) was used for 1hr at room temperature.

      The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
      Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
      All lanes : Anti-alpha Tubulin antibody - Loading Control (ab89984) at 1 µg/ml

      Lane 1 : Liver (Human) Tissue Lysate
      Lane 2 : Liver (Mouse) Tissue Lysate
      Lane 3 : Liver (Rat) Tissue Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab205721) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 52 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab89984 overnight at 4°C. Antibody binding was detected using ab205721, and visualised using ECL development solution ab133406.

    • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
      Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab205721)
      All lanes : No Primary Antibody

      Lane 1 : Liver (Human) Tissue Lysate
      Lane 2 : Liver (Mouse) Tissue Lysate
      Lane 3 : Liver (Rat) Tissue Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab205721) at 1/2000 dilution

      Performed under reducing conditions.

      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with only the secondary antibody (ab205721), and visualised using ECL development solution ab133406.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab205721? Please let us know so that we can cite the reference in this datasheet.

    ab205721 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Test ab205721 - Goat Anti-Chicken IgY H&L (HRP)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Average specificity.
    Average sensitivity.
    WB conditions:
    - Cell lisates: 20 μg protein
    - non-reducing
    - Primary antibody: Chicken anti-alpha Tubulin. ab89984. Abcam. Dilution 1:1000
    - Secondary antibody: 1:6000
    - Developer: ECL Prime, Amersham, GE Helathcare Life Sciences, Product code RPN2232
    - Exposure time: 1 second, 2 exposures: 3th film out 4 4th film out 4.

    Abcam user community

    Verified customer

    Submitted Feb 01 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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