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Secondary antibodies anti-Chicken IgY Enzyme HRP
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Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (28)

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Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

Key features and details

  • Goat Anti-Chicken IgY H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Enzyme
    • HRP

Associated products

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

Our Abpromise guarantee covers the use of ab6877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/10000.
Dot blot Use at an assay dependent dilution.
ELISA 1/50000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/20000. PubMed: 16543490

Images

  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)Lichtenfels et al PLoS One. 2012;7(7):e41345. doi: 10.1371/journal.pone.0041345. Epub 2012 Jul 20. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Representative Western blot analysis for the selected panel of differentially expressed targets in the various T cell subsets.

    Western blot analyses were performed by serially probing sets of 3 membranes per donor. The expression levels of GDIR1, GSTO1, LIMS1 and PROF1 were analyzed blot 1 (upper panel), GDIR2, PRDX2 and THIO on blot 2 (middle panel) and SODM on blot 3 (lower level) of the respective membrane set.

    The relative expression levels were defined by densitometric quantification normalized to the corresponding beta-actin signals, co-detected on each membrane.

    Aliquots of 15 µg total lysate per sample/lane were loaded onto Tris-Tricine gels (16%T/2.5%C) and subsequently separated. Lysates generated form untreated CD45RA+ and CD45RO+ T cells were throughout the set of membranes loaded onto the lanes 1 and 3 respectively, whereas lysates generated from the corresponding T cell subsets exposure for 3 h to 5 µM H2O2 were loaded onto the lanes 2 and 4 as indicated.

    The protein entry names of the targets (left) and their corresponding molecular weight (right) are listed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

    IHC image of beta Actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab13822, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6877, 1/500 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

    ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

    ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (28)

    Publishing research using ab6877? Please let us know so that we can cite the reference in this datasheet.

    ab6877 has been referenced in 28 publications.

    • Cui L  et al. Overexpression of CCDC69 activates p14ARF/MDM2/p53 pathway and confers cisplatin sensitivity. J Ovarian Res 12:4 (2019). PubMed: 30651135
    • Shrestha A  et al. The metacaspase Yca1 maintains proteostasis through multiple interactions with the ubiquitin system. Cell Discov 5:6 (2019). PubMed: 30675380
    • Lu L  et al. Human Antimicrobial RNases Inhibit Intracellular Bacterial Growth and Induce Autophagy in Mycobacteria-Infected Macrophages. Front Immunol 10:1500 (2019). PubMed: 31312205
    • Sopova JV  et al. RNA-binding protein FXR1 is presented in rat brain in amyloid form. Sci Rep 9:18983 (2019). PubMed: 31831836
    • Kim HJ & Han SJ Anodal Transcranial Direct Current Stimulation Provokes Neuroplasticity in Repetitive Mild Traumatic Brain Injury in Rats. Neural Plast 2017:1372946 (2017). PubMed: 28770112
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Human iPSC-derived neurons MAP2

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Protein extracts from human iPSC-derived neurons were separated on the SDS PAGE and immunoblotted with MAP2 antibody (ab5392, 1:40 000 dilution). Secondary goat anti chicken antibody (ab6877) were used at 1:10 000 dilution.

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 30 2020

    Question

    Is AB 6877 IGG or IGY? The website says IGY so a customer ordered this product from us. The lable on the vial however says IGG but has the correct item number (6877). Was this an error on the label or the website? Thanks much

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2002

    Answer

    This antibody is a goat IgG anti-chicken IgY. We apologise for any mistakes on the vial label.

    Read More

    Abcam Scientific Support

    Answered on Sep 09 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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