Goat Anti-Chicken IgY H&L (HRP) (ab6877)
- Datasheet
- Specific References (23)
- Protocols
Overview
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Product nameGoat Anti-Chicken IgY H&L (HRP)
See all IgY secondary antibodies -
Host speciesGoat
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Target speciesChicken
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Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
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Immunogen
chicken IgY whole molecule
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ConjugationHRP
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Conjugation notesHorseradish Peroxidase (HRP)
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab6877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000 - 1/10000. | |
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | 1/50000. | |
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| Immunomicroscopy | Use at an assay dependent dilution. | |
| WB | 1/2000 - 1/20000. PubMed: 16543490 |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6877) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
References
This product has been referenced in:
- Kim HJ & Han SJ Anodal Transcranial Direct Current Stimulation Provokes Neuroplasticity in Repetitive Mild Traumatic Brain Injury in Rats. Neural Plast 2017:1372946 (2017). Read more (PubMed: 28770112) »
- Bobeck EA et al. Oral antibodies to human intestinal alkaline phosphatase reduce dietary phytate phosphate bioavailability in the presence of dietary 1a-hydroxycholecalciferol. Poult Sci 95:570-80 (2016). Read more (PubMed: 26666254) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"