Goat Anti-Chicken IgY H&L (HRP) (ab97135)
Key features and details
- Goat Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Chicken IgY H&L (HRP)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
|
| IHC-P |
1/200 - 1/5000.
|
|
| ELISA | (1) |
1/10000 - 1/100000. (Primary).
|
| WB | (1) |
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000
|
| Notes |
|---|
|
ICC
Use at an assay dependent dilution. |
|
IHC-P
1/200 - 1/5000. |
|
ELISA
1/10000 - 1/100000. (Primary). |
|
WB
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000 |
Images
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Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 90 seconds -
Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)This image is courtesy of an anonymous Abreview.All lanes : Anti-Vimentin antibody (ab24525)
All lanes : Mouse cardiac fibroblasts
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab97135) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 seconds
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Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)Ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). Ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.
This image is courtesy of an anonymous Abreview.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab97135 has been referenced in 24 publications.
- Uematsu K et al. Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors. Int J Mol Sci 22:N/A (2021). PubMed: 33671612
- Brady NJ et al. Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer. Nat Commun 12:3372 (2021). PubMed: 34099734
- Chen P et al. Structural basis for CSPG4 as a receptor for TcdB and a therapeutic target in Clostridioides difficile infection. Nat Commun 12:3748 (2021). PubMed: 34145250
- Mason B et al. Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O-GlcNAcylation. Cell Mol Life Sci 77:2605-2620 (2020). PubMed: 31560077
- Taglialegna A et al. The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers. NPJ Biofilms Microbiomes 6:15 (2020). PubMed: 32221298