Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118)

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Overview

  • Product name
    Goat Anti-Chicken IgY H&L (HRP) preadsorbed
    See all IgY secondary antibodies
  • Host species
    Goat
  • Target species
    Chicken
  • Tested applications
    Suitable for: WB, Immunomicroscopy, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native chicken IgY (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/2500.
Dot blot Use at an assay dependent dilution.
ELISA 1/20000 - 1/100000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References

ab7118 has not yet been referenced specifically in any publications.

Publishing research using ab7118? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Where is the questionnaire that I should fill out? If it is the note with product number and lot number, I already filled that out in my previous inquiry.

Best regards,

Thank you for your quick response.

The Questionnaire has been attached to my first response as a word document but I have reattached it to this e-mail as well.

Please could you add further information to the Questionnaire? I am part...

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1) Abcam product code ab7118



2) Abcam order reference number or product batch number lot nr GR27997-6



3) Description of the problem: unspecific bands on western, with using only the secondary antibody (no p...

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Thank you for your enquiry regardingab7118 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Though you have kindly provided some details, it wo...

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Lot number: GR27997-6
Enquiry: Hi, I experience problems with the secondary antibody: I see bands on the blot, without adding primary antibody. This would indicate unspecific binding of secondary antibody to the proteins on the membrane. How do I ...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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