Key features and details
- Goat polyclonal Secondary Antibody to Chicken IgY - H&L, pre-adsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat Anti-Chicken IgY H&L preadsorbed
See all IgY secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Chicken IgY - H&L, pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Cow, Horse, Human, Mouse, Pig, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Other Immunogen Type corresponding to Chicken IgY.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab97141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/100 - 1/10000. ELISA-Coating 1/100 - 1/500; ELISA-Primary 1/1000 - 1/10000|
|WB||1/1000 - 1/30000. WB-Colorimetric 1/1000 - 1/10000; WB-Chemiluminescent 1/1000 - 1/30000|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97141 has not yet been referenced specifically in any publications.