Overview

  • Product name

    Goat Anti-Chicken IgY H&L preadsorbed
    See all IgY secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L, pre-adsorbed
  • Host species

    Goat
  • Target species

    Chicken
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Chicken IgY.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/100 - 1/10000. ELISA-Coating 1/100 - 1/500; ELISA-Primary 1/1000 - 1/10000
WB 1/1000 - 1/30000. WB-Colorimetric 1/1000 - 1/10000; WB-Chemiluminescent 1/1000 - 1/30000

References

ab97141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.  We have only tested these Peroxiredoxin antibodies under reducing conditions in WB.  They may be suitable for use under non-reducing conditions as well, but this has not been validated in house.  The unconjugated secondary antibody ab97141 will react with the primary antibody ab62391 and is suitable for use in WB.  You can detect this secondary antibody by either conjugating it with one of our EasyLink conjugating kits, or using an additional anti-goat secondary antibody.  Alternatively, you could use the equivalent HRP conjugated anti-Chicken IgY secondary antibody ab97150,  I hope this helps, please let me know if you need any additional information. 

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Answer

Thank you for contacting us. Ab97141 is an un-conjugated secondary antibody to Chicken IgY. In order of using this antibody in different applications, you would need a fluorophore conjugated anti goat antibody. However it will depend on the application of use. We also have same antibody with different conjugates in catalogue. The catalogue numbers are ab97142 ab97149 ab97150 Please have a look at the datasheets of these and buy as required. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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