Key features and details
- Goat Anti-Donkey IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot
Related conjugates and formulations
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/40000 - 1/160000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6987 has been referenced in 2 publications.
- Aldahl J et al. Aberrant activation of hepatocyte growth factor/MET signaling promotes ß-catenin-mediated prostatic tumorigenesis. J Biol Chem 295:631-644 (2020). PubMed: 31819003
- Mi J et al. Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumor aggressiveness in the murine prostate. J Biol Chem 293:20123-20136 (2018). PubMed: 30401749