Goat Anti-Donkey IgG H&L (Biotin) (ab6987)

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Key features and details

  • Goat Anti-Donkey IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot

Conjugates logo Related conjugates and formulations

DyLight® 488 DyLight® 550 DyLight® 594 HRP Texas Red ® Unconjugated

Overview

  • Product name

    Goat Anti-Donkey IgG H&L (Biotin)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Donkey

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details

  • Immunogen

    Full length native donkey IgG (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
WB
ELISA
Immunomicroscopy
Dot blot
Notes
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/40000 - 1/160000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab6987? Please let us know so that we can cite the reference in this datasheet.

ab6987 has been referenced in 2 publications.

  • Aldahl J  et al. Aberrant activation of hepatocyte growth factor/MET signaling promotes ß-catenin-mediated prostatic tumorigenesis. J Biol Chem 295:631-644 (2020). PubMed: 31819003
  • Mi J  et al. Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumor aggressiveness in the murine prostate. J Biol Chem 293:20123-20136 (2018). PubMed: 30401749

Customer reviews and Q&As

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Question

Has the biotinylated Goat anti-Donkey antibody listed in in your online catalog been cross absorbed against any other proteins?

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Answer

It has not been absorbed against any other proteins.

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