Goat Anti-Donkey IgG H&L (Biotin) (ab6987)
- Datasheet
- References
- Protocols
Overview
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Product nameGoat Anti-Donkey IgG H&L (Biotin)
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesDonkey
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Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
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Immunogen
Full length native donkey IgG (purified).
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ConjugationBiotin
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityAffinity purified
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Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
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Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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ClonalityPolyclonal
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IHC-P | ||
| IHC-Fr | ||
| WB | ||
| ELISA | ||
| Immunomicroscopy | ||
| Dot blot |
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/40000 - 1/160000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/40000 - 1/160000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Datasheets and documents
References
ab6987 has not yet been referenced specifically in any publications.
