Goat Anti-Donkey IgG H&L (Biotin) (ab6987)
Key features and details
- Goat Anti-Donkey IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blot
Related conjugates and formulations
Overview
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Product name
Goat Anti-Donkey IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Donkey -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details -
Immunogen
Full length native donkey IgG (purified).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | ||
IHC-P | ||
IHC-Fr | ||
WB | ||
ELISA | ||
Immunomicroscopy | ||
Dot blot |
Notes |
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ELISA: 1/40000 - 1/160000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab6987 has been referenced in 2 publications.
- Aldahl J et al. Aberrant activation of hepatocyte growth factor/MET signaling promotes ß-catenin-mediated prostatic tumorigenesis. J Biol Chem 295:631-644 (2020). PubMed: 31819003
- Mi J et al. Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumor aggressiveness in the murine prostate. J Biol Chem 293:20123-20136 (2018). PubMed: 30401749