Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small)) (ab30813)

Overview

  • Product name

    Goat F(ab) Anti-Mouse IgG H&L (1.4nm Gold (ultra-small))
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: WB, Electron Microscopy, ICCmore details
  • Immunogen

    Mouse IgG whole molecule.

  • Conjugation

    Gold 1.4nm (ultra-small)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0536% PBS, 0.87% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Chromatographically purified through gel filtration columns.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

     

    This product is a newly developed ultra small gold immunoprobe. It is stable to wide ranges of pH and ionic strength. It is not radioactive or carcinogenic. Normal methologies may be used successfully with this product. The concentration of antibody and gold is similar to other commmercial preparations of colloidal gold antibodies. Therefore similar dilutions and blocking agents are appropriate.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

References

ab30813 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. Unfortunately, the affinity constants for ab30812 and ab30813 have not been measured. The gold particle is linked covalently: it is conjugated via a peripheral maleimido- group which is introduced into a peripheral coordinated ligand, and this is reacted with a thiol in the hinge region of the IgG or Fab' fragments, generated by selective reduction; the resulting bond is stable to reasonaby wide variations in pH and buffer conditions. The location of this gold particle may be observed by scanning transmission electron microscopy (STEM). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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