Product nameGoat F(ab) Anti-Mouse IgG H&L
See all IgG secondary antibodies
DescriptionGoat F(ab) polyclonal Secondary Antibody to Mouse IgG - H&L
Tested applicationsSuitable for: WB, ICC/IF, IHC, ELISAmore details
Mouse IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).”
Our Abpromise guarantee covers the use of ab6668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|ICC/IF||1/1000 - 1/5000.|
|IHC||1/1000 - 1/5000.|
SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) ab6668.
Lane 1: Non-reduced Goat Fab Anti-Mouse.
Lane 2: Opal Prestained Ladder.
Lane 3: Reduced Goat Fab Anti-Mouse.
Load: 1.0 µg.
Coomassie stained and imaged.
Dot Blot results of Goat Fab Anti-Mouse IgG Antibody ab6668.
Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.
Blocked for 30 min at RT.
Primary Antibody: Goat Fab Anti-Mouse IgG at 1 µg/mL for 1 hr at RT.
Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.
Imaged with BioRad ChemiDoc, Chemi filter.
This product has been referenced in:
- Anderson SE et al. Determination of a Critical Size Threshold for Volumetric Muscle Loss in the Mouse Quadriceps. Tissue Eng Part C Methods 25:59-70 (2019). Read more (PubMed: 30648479) »
- Studentsova V et al. Insulin Receptor deletion in S100a4-lineage cells accelerates age-related bone loss. Bone Rep 10:100197 (2019). Read more (PubMed: 30805422) »