• Product name
    Goat F(ab) Anti-Mouse IgG H&L
    See all IgG secondary antibodies
  • Description
    Goat F(ab) polyclonal Secondary Antibody to Mouse IgG - H&L
  • Host species
  • Target species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC, ELISAmore details
  • Immunogen

    Mouse IgG whole molecule


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).”
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab6668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
ICC/IF 1/1000 - 1/5000.
IHC 1/1000 - 1/5000.
ELISA 1/50000.


This product has been referenced in:
  • Li C  et al. The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining. Nat Commun 9:1006 (2018). Read more (PubMed: 29520062) »
  • Tong WY  et al. Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology 16:38 (2018). Read more (PubMed: 29653579) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

As requested I have included with this email our Mouse on Mouse staining tips. For blocking of endogenous IgG we recommend using Goat F (ab) polyclonal Secondary Antibody to Mouse IgG - H&L (ab6668). More i...

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Thank you for contacting us.

The forms which you have requested have been created and are now available on the webpages for each product. For your convenience I have also include these with this email.

I hope this information ...

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I have spoken with the group in charge of creating product SDS forms on your behalf. They have informed me that they have created this form and it will be posted on the public webpage for the product soon. Read More

Thank you for contacting us, I apologize for the delay in my reply. I would recommend ab114130 for your purposes. The immunogen for this antibody shows no significant similarity to PDGFA, and it is slightly less likely to react with mouse than our othe...

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Thank you for your reply.

The pretreatment can be performed using a 1:100 dilution of ab6668 for 1 hour just after the serum block. This should be sufficient for blocking endogenous mouse IgGs in your tissue sections. Please let me know if yo...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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Thank you for your calls today, and it was a pleasure speaking to you. I apologize for the delay in getting this email out to you.

As we discussed,I would recommend using a secondary antibody that lacks the Fc portion (also called "const...

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Thank you for contacting us.

Indeed, a mouse-on mouse staining can be quite tricky; however, it is not impossible. The background will depend on the fixation method ( perfusion fixed tissue vs. normally fixed tissue), on the tissue itself ( ...

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Thanks for keeping me updated abouttheprogress. I'm so glad to hear that ab7778 gave positive results!

Please let me know if there is anything else that you might need from me in the future. Have a wonderful weekend!

Thank you for getting back to me and for sending these details.

I have issued credit note number *** for the collagen and elastin antibodies. The purchase prices of these antibodies will be refunded back to your credit card pending approval f...

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1-10 of 16 Abreviews or Q&A


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