Product nameGoat F(ab) Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Tested applicationsSuitable for: Dot blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IFmore details
Mouse IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule
Our Abpromise guarantee covers the use of ab6822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|IHC||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|ICC/IF||1/1000 - 1/5000.|
ab6822 has not yet been referenced specifically in any publications.