Goat F(ab) Anti-Mouse IgG H&L (FITC) (ab6669)

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Overview

  • Product name

    Goat F(ab) Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details

  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Flow Cyt Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:

  • Kochegarov A  et al. A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos. J Biomed Sci 20:20 (2013). Read more (PubMed: 23522091) »
  • Mogilenko DA  et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). Read more (PubMed: 22271762) »
See all 3 Publications for this product

Publishing research using ab6669? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Good fluorescence intensity

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
A431 cells were corrected using accutase.
After incubation with an anti-EGFR antibody, these cells were stained with this anti-mouse IgG-FITC antibody for 30 min at RT.
isotype control (Blue) was mouse IgG.
anti-EGFR antibody (Red)
Dilution: 1/1000 (1.0x10^6 cells/mL)

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Submitted Jul 07 2017

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