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Immunology Immunoglobulins Heavy Chain IgG
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Goat F(ab) Anti-Mouse IgG H&L (FITC) (ab6669)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Key features and details

  • Goat F(ab) Anti-Mouse IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA

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Overview

  • Product name

    Goat F(ab) Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • FITC

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6669 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/1000 - 1/5000.
    Flow Cyt (1)
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    ELISA
    1/10000 - 1/50000.
    Notes
    ICC/IF
    1/1000 - 1/5000.
    Flow Cyt
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    ELISA
    1/10000 - 1/50000.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab6669? Please let us know so that we can cite the reference in this datasheet.

    ab6669 has been referenced in 3 publications.

    • Kochegarov A  et al. A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos. J Biomed Sci 20:20 (2013). PubMed: 23522091
    • Mogilenko DA  et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). PubMed: 22271762
    • Mogilenko DA  et al. Modified low density lipoprotein stimulates complement C3 expression and secretion via liver X receptor and toll-like receptor 4 activation in human macrophages. J Biol Chem : (2011). PubMed: 22194611

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Good fluorescence intensity

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    A431 cells were corrected using accutase.
    After incubation with an anti-EGFR antibody, these cells were stained with this anti-mouse IgG-FITC antibody for 30 min at RT.
    isotype control (Blue) was mouse IgG.
    anti-EGFR antibody (Red)
    Dilution: 1/1000 (1.0x10^6 cells/mL)

    Abcam user community

    Verified customer

    Submitted Jul 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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