Product nameGoat F(ab) Anti-Mouse IgG H&L (FITC)
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Tested applicationsSuitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
Mouse IgG whole molecule
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Our Abpromise guarantee covers the use of ab6669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Flow Cyt||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|ELISA||1/10000 - 1/50000.|
This product has been referenced in:
- Kochegarov A et al. A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos. J Biomed Sci 20:20 (2013). Read more (PubMed: 23522091) »
- Mogilenko DA et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). Read more (PubMed: 22271762) »