Overview

  • Product name

    Goat F(ab) Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. It was tested against anti goat F(c) fragment to verify no contaminating goat F(c) was present in the fraction. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Constituent: 0.0014% Zinc chloride

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notes

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/500 - 1/2500.
ELISA 1/10000 - 1/100000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

Images

  • Ab6823 was used with the primary antibody ab15263 in IHC-P. See Abreview on ab15263.

References

This product has been referenced in:

  • Sakamoto S  et al. Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin. Talanta 194:731-736 (2019). Read more (PubMed: 30609599) »
  • Yusakul G  et al. An indirect competitive enzyme-linked immunosorbent assay toward the standardization of Pueraria candollei based on its unique isoflavonoid, kwakhurin. Fitoterapia 133:23-28 (2018). Read more (PubMed: 30572086) »
See all 10 Publications for this product

Customer reviews and Q&As

Question
Answer

The product you are looking for is ab6668, Goat F(ab) polyclonal Secondary Antibody to Mouse IgG without HRP.

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