Goat F(ab) Anti-Rabbit IgG H&L (1.4nm Gold (ultra-small) + FITC) (ab33110)

Overview

  • Product name

    Goat F(ab) Anti-Rabbit IgG H&L (1.4nm Gold (ultra-small) + FITC)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Electron Microscopy, ICC/IF, WBmore details
  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation

    FITC + Gold 1.4nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0536% PBS, 0.87% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Chromatographically purified through gel filtration columns.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

     

    This product is a revolutionary new immunocytochemical reagent. This unique antibody probe contains a molecular label which contains both the 1.4nm ultra-small gold particle and fluorescein, enabling both fluorescence and electron microscope observation of a sample stained in a single labelling procedure. This probe is smaller than a whole IgG molecule, does not aggregate, and fluorescence quenching due to the goold particle is negligible. Normal methologies may be used successfully with this product. Due to some quenching of fluorescence by the gold particle, slightly higher concentrations of antibody are recommended for incubations. A blocking agent of 5% non-fat dried milk has been found to reduce background in some cases: this should be used before incubation with probe (in standard wash/blocking steps), and additionally, the Fluorescein-ultra-small gold probe should be diluted in a solution also containing 5% non-fat dried milk before it is applied.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References

This product has been referenced in:

  • Tang Y  et al. Low concentrations of bilirubin inhibit activation of hepatic stellate cells in vitro. Mol Med Rep 15:1647-1653 (2017). Read more (PubMed: 28260031) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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