Goat F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171)
Key features and details
- Goat F(ab) Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Overview
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Product name
Goat F(ab) Anti-Rabbit IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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WB |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
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Electron Microscopy |
Use at an assay dependent dilution.
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IHC |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab7171 has been referenced in 8 publications.
- Lejkowska R et al. Preclinical Evaluation of Long-Term Neuroprotective Effects of BDNF-Engineered Mesenchymal Stromal Cells as Intravitreal Therapy for Chronic Retinal Degeneration in Rd6 Mutant Mice. Int J Mol Sci 20:N/A (2019). PubMed: 30759764
- Cassetta L et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell 35:588-602.e10 (2019). PubMed: 30930117
- Falcão AM et al. Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis. Nat Med 24:1837-1844 (2018). PubMed: 30420755
- Makieva S et al. The preterm cervix reveals a transcriptomic signature in the presence of premature prelabor rupture of membranes. Am J Obstet Gynecol 216:602.e1-602.e21 (2017). PubMed: 28209491
- Hindle P et al. The Infrapatellar Fat Pad as a Source of Perivascular Stem Cells with Increased Chondrogenic Potential for Regenerative Medicine. Stem Cells Transl Med 6:77-87 (2017). PubMed: 28170170
- West A et al. Npas4 is activated by melatonin, and drives the clock gene Cry1 in the ovine pars tuberalis. Mol Endocrinol 27:979-89 (2013). PubMed: 23598442
- Rivera HM et al. Estradiol increases the anorexia associated with increased 5-HT(2C) receptor activation in ovariectomized rats. Physiol Behav : (2011). PubMed: 21889523
- Bayne RA et al. Prostaglandin E2 as a regulator of germ cells during ovarian development. J Clin Endocrinol Metab 94:4053-60 (2009). PubMed: 19602557