Goat F(ab) Anti-Rabbit IgG H&L (HRP) (ab7171)

Submit a review Q&A (1)References (8)

Key features and details

  • Goat F(ab) Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

Overview

  • Product name

    Goat F(ab) Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

  • Conjugation notes

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.
Notes
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (8)

Publishing research using ab7171? Please let us know so that we can cite the reference in this datasheet.

ab7171 has been referenced in 8 publications.

  • Lejkowska R  et al. Preclinical Evaluation of Long-Term Neuroprotective Effects of BDNF-Engineered Mesenchymal Stromal Cells as Intravitreal Therapy for Chronic Retinal Degeneration in Rd6 Mutant Mice. Int J Mol Sci 20:N/A (2019). PubMed: 30759764
  • Cassetta L  et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell 35:588-602.e10 (2019). PubMed: 30930117
  • Falcão AM  et al. Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis. Nat Med 24:1837-1844 (2018). PubMed: 30420755
  • Makieva S  et al. The preterm cervix reveals a transcriptomic signature in the presence of premature prelabor rupture of membranes. Am J Obstet Gynecol 216:602.e1-602.e21 (2017). PubMed: 28209491
  • Hindle P  et al. The Infrapatellar Fat Pad as a Source of Perivascular Stem Cells with Increased Chondrogenic Potential for Regenerative Medicine. Stem Cells Transl Med 6:77-87 (2017). PubMed: 28170170
  • West A  et al. Npas4 is activated by melatonin, and drives the clock gene Cry1 in the ovine pars tuberalis. Mol Endocrinol 27:979-89 (2013). PubMed: 23598442
  • Rivera HM  et al. Estradiol increases the anorexia associated with increased 5-HT(2C) receptor activation in ovariectomized rats. Physiol Behav : (2011). PubMed: 21889523
  • Bayne RA  et al. Prostaglandin E2 as a regulator of germ cells during ovarian development. J Clin Endocrinol Metab 94:4053-60 (2009). PubMed: 19602557

Customer reviews and Q&As

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Submit a review Submit a question

Question

How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
Customer concern cross reaction of secondary anibody.
Which secondary antibody you suggest since their brain sample also from mouse.
Thanks a lot for your assistance.
Best regards,

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Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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