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  1. Link

    goat-fab2-human-igg-fab2-hrp-ab98535.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP) (ab98535)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (7)

Product price, shipping and contact information

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Promotion Information

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Find out more.

Key features and details

  • Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

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Overview

  • Product name

    Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Enzyme
    • HRP

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB (1)
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000).
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab98535? Please let us know so that we can cite the reference in this datasheet.

ab98535 has been referenced in 7 publications.

  • Passariello M  et al. Novel human neutralizing mAbs specific for Spike-RBD of SARS-CoV-2. Sci Rep 11:11046 (2021). PubMed: 34040046
  • Tan XW  et al. Rapid and specific detection of oxidized LDL/ß2GPI complexes via facile lateral flow immunoassay. Heliyon 6:e04114 (2020). PubMed: 32551380
  • Seabright GE  et al. Networks of HIV-1 Envelope Glycans Maintain Antibody Epitopes in the Face of Glycan Additions and Deletions. Structure 28:897-909.e6 (2020). PubMed: 32433992
  • Passariello M  et al. Isolation of Two Novel Human Anti-CTLA-4 mAbs with Intriguing Biological Properties on Tumor and NK Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32781690
  • Passariello M  et al. Novel Human Anti-PD-L1 mAbs Inhibit Immune-Independent Tumor Cell Growth and PD-L1 Associated Intracellular Signalling. Sci Rep 9:13125 (2019). PubMed: 31511565
  • Sasso E  et al. Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies. MAbs 10:1060-1072 (2018). PubMed: 29995563
  • Oaks M  et al. Xenoreactive antibodies and latent fibrin formation in VAD and cardiac transplant recipients can confound the detection and measurement of anti-AT1R antibodies. Am J Transplant 18:2763-2771 (2018). PubMed: 29603642

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Specific 2nd antibody does not react with Fc

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
This antibody is specific to Fab, it does not react with Fc, tested in western blotting loaded with human IgG and human Fc-fusion protein without primary antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 14 2015

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