Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP) (ab98535)

Reviews (1)Q&A (2)References (2)

Key features and details

  • Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab98535 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC Use at an assay dependent dilution.
    IHC-P 1/200 - 1/2000.
    ELISA 1/10000 - 1/100000. (Primary).
    WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab98535? Please let us know so that we can cite the reference in this datasheet.

    ab98535 has been referenced in 2 publications.

    • Passariello M  et al. Novel Human Anti-PD-L1 mAbs Inhibit Immune-Independent Tumor Cell Growth and PD-L1 Associated Intracellular Signalling. Sci Rep 9:13125 (2019). PubMed: 31511565
    • Sasso E  et al. Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies. MAbs 10:1060-1072 (2018). PubMed: 29995563

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Specific 2nd antibody does not react with Fc

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    This antibody is specific to Fab, it does not react with Fc, tested in western blotting loaded with human IgG and human Fc-fusion protein without primary antibody.

    Abcam user community

    Verified customer

    Submitted Oct 14 2015

    Question

    Will it bind to the heavy chain portion of the Fab'2 fragment of human IgG? I'm expressing only the heavy chain portion of the Fab'2 fragment, and the antibody won't work unless it recognizes this chain.

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    Answer

    This antibody will react with the heavy chain portion of the Fab’2 fragment. Please bear in mind that this reaction will not be as efficient, as only a portion of the polyclonal antibody population will be against this portion, so you may need to use a higher concentration.

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    Question

    Product code: 98535

    Inquiry: Dear Sir/Madam, I represent biotech company. We are focused on development of monoclonal antibodies. We are preparing for clinical trials in patients with rheumatoid arthritis. We will assess HACA ( human antichimeric antibodies) in our patients using ELISA. I would like to ask about completed kits for such assessments. I found out on Your website that You have a wide range of kits including various antibodies. We are interested in rabbit anti-human IgG F(ab')2 specific peroxidise conjugated antibody and rabbit anti-human IgG (whole molecule). Could You, please, inform us if You are provided with such antibodies and what will be the cost for 500 assessments or could You propose me another type of antibodies for our assessments. We are interested as well in singulair antibodies as in completed kits. I will be very grateful for You help. Your sincerely,

    Read More
    Answer

    Thank you for your inquiry.

    I am sorry to confirmwe do not have antibodies or kits for detection of the human IgG F(ab')2 fragment (though we do have several to human IgG F(ab') fragment).

    We have the following rabbit anti-human IgG antibodies, however I am sorry these are not peroxidase conjugated. I can recommend to review the online datasheets for further information and a list of the guaranteed applications for each individual antibody and prices.

    https://www.abcam.com/index.html?pageconfig=searchresults&search=rabbit human IgG&pt=1&sk=targ&sv=Human IgG&sn=Human IgG&l=2&fViewMore=1

    Please note that regrettablyour products are not accredited for clinical and diagnostic use. They are for research purposes only.

    We aim to provide as much help as possible to customers and provide a wide range of products. I am sorry we do not have theitems you require in the catalogon this occasion. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:

    http://www.biocompare.com
    http://www.biocompare.com/ProductCategories/2045/Antibodies.html


    I hope this information will be helpful to you. Please do not hesitate to contact me if you have any further questions.

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