Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP) (ab98535)

Abreviews (1)Q&A (2)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000).

References

ab98535 has not yet been referenced specifically in any publications.

Publishing research using ab98535? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Specific 2nd antibody does not react with Fc

 Excellent
Abreviews
Application
Western blot
This antibody is specific to Fab, it does not react with Fc, tested in western blotting loaded with human IgG and human Fc-fusion protein without primary antibody.
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Submitted Oct 14 2015

Will it bind to the heavy chain portion of the Fab'2 fragment of human IgG? I'm expressing only the heavy chain portion of the Fab'2 fragment, and the antibody won't work unless it recognizes this chain.

This antibody will react with the heavy chain portion of the Fab’2 fragment. Please bear in mind that this reaction will not be as efficient, as only a portion of the polyclonal antibody population will be against this portion, so you may need t...

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Product code: 98535

Inquiry: Dear Sir/Madam, I represent biotech company. We are focused on development of monoclonal antibodies. We are preparing for clinical trials in patients with rheumatoid arthritis. We will assess HACA ( human antichi...

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Thank you for your inquiry.

I am sorry to confirmwe do not have antibodies or kits for detection of the human IgG F(ab')2 fragment (though we do have several to human IgG F(ab') fragment).

We have the following rabbit anti-human I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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