Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

Abreviews (1) Submit a Question Specific References (2)

Overview

  • Product name
    Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Human
  • Tested applications
    Suitable for: Flow Cytmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.

References

This product has been referenced in:
  • Ouisse LH  et al. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnol 17:3 (2017). Read more (PubMed: 28081707) »
  • Sun J  et al. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nat Commun 8:477 (2017). Read more (PubMed: 28883531) »

See all 2 Publications for this product

Publishing research using ab98596? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

Detecting binding of remicade to CHO cells expressing membrane bound human TNFa

 Good
Abreviews
Application
Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5ug/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
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Submitted Apr 25 2017

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