Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Tested applications
Suitable for: Flow Cytmore details -
Minimal
cross-reactivity
Mouse, Rat more details -
Conjugation
Phycoerythrin. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab98596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50 - 1/200. |
Images
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Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10 μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5 min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5 µg/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.
PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
Datasheets and documents
References
This product has been referenced in:
- Ouisse LH et al. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnol 17:3 (2017). Read more (PubMed: 28081707) »
- Sun J et al. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nat Commun 8:477 (2017). Read more (PubMed: 28883531) »
