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  1. Link

    goat-fab2-human-igg-fc-pe-pre-adsorbed-ab98596.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

  • Datasheet
Reviews (1) Submit a question References (8)

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Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

    Key features and details

    • Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Flow Cyt

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Human
    • Tested applications

      Suitable for: Flow Cytmore details
    • Minimal
      cross-reactivity


      Mouse, Rat
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 0.2% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Human
      • IgG
      • Fluorophore
      • Phycoerythrin

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab98596 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (1)
    1/50 - 1/200.
    Notes
    Flow Cyt
    1/50 - 1/200.

    Images

    • Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
      Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

      Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

      CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10 μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5 min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5 µg/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

      PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (8)

    Publishing research using ab98596? Please let us know so that we can cite the reference in this datasheet.

    ab98596 has been referenced in 8 publications.

    • Mutoh T  et al. Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis. Nat Commun 11:1253 (2020). PubMed: 32152303
    • Yu X  et al. Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity. Cancer Cell 37:850-866.e7 (2020). PubMed: 32442402
    • Wan J  et al. Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infection. Cell Rep 32:107918 (2020). PubMed: 32668215
    • Liu X  et al. A universal dual mechanism immunotherapy for the treatment of influenza virus infections. Nat Commun 11:5597 (2020). PubMed: 33154358
    • Rengarajan S  et al. Dynamic immunoglobulin responses to gut bacteria during inflammatory bowel disease. Gut Microbes 11:405-420 (2020). PubMed: 31203722
    • Zhang Q  et al. A Novel mTORC1/2 Inhibitor (MTI-31) Inhibits Tumor Growth, Epithelial-Mesenchymal Transition, Metastases, and Improves Antitumor Immunity in Preclinical Models of Lung Cancer. Clin Cancer Res 25:3630-3642 (2019). PubMed: 30796032
    • Ouisse LH  et al. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnol 17:3 (2017). PubMed: 28081707
    • Sun J  et al. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nat Commun 8:477 (2017). PubMed: 28883531

    Customer reviews and Q&As

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    Submit a review Submit a question

    Detecting binding of remicade to CHO cells expressing membrane bound human TNFa

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5ug/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

    PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 25 2017

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