Overview

  • Product name

    Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Human
  • Tested applications

    Suitable for: Flow Cytmore details
  • Minimal
    cross-reactivity


    Mouse, Rat more details
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.

Images

  • Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)

    CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10 μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5 min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5 µg/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

    PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.

References

This product has been referenced in:

  • Ouisse LH  et al. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnol 17:3 (2017). Read more (PubMed: 28081707) »
  • Sun J  et al. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nat Commun 8:477 (2017). Read more (PubMed: 28883531) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5ug/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.

PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.

Abcam user community

Verified customer

Submitted Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up