Key features and details
- Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
Product nameGoat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Cow, Horse, Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Human IgG (purified).
ConjugationPE. Ex: 488nm, Em: 575nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
General notesCan be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Our Abpromise guarantee covers the use of ab7006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/200.|
|IHC-Fr||1/50 - 1/200.|
|Immunomicroscopy||1/50 - 1/200.|
|Flow Cyt||1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7006 has been referenced in 2 publications.
- Alvarez-Cienfuegos A et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). PubMed: 27345490
- Tiwari A et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). PubMed: 24510768