Key features and details
- Goat F(ab')2 Anti-Human IgG - H&L, pre-adsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat F(ab')2 Anti-Human IgG - H&L, pre-adsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Our Abpromise guarantee covers the use of ab98577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).|
|WB||1/1000 - 1/30000. (Colorimetric 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98577 has not yet been referenced specifically in any publications.