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Goat F(ab')2 Anti-Human IgG - H&L, pre-adsorbed (ab98577)

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Key features and details

  • Goat F(ab')2 Anti-Human IgG - H&L, pre-adsorbed
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat F(ab')2 Anti-Human IgG - H&L, pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Human

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Mouse, Rat more details

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
WB
1/1000 - 1/30000. (Colorimetric 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).
WB
1/1000 - 1/30000. (Colorimetric 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab98577? Please let us know so that we can cite the reference in this datasheet.

ab98577 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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