Key features and details
- Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, WB, ELISA, IHC-P
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline phosphatase.
Our Abpromise guarantee covers the use of ab98656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|WB||1/1000 - 1/10000. by chemiluminescent detection|
|ELISA||1/1000 - 1/10000. as primary|
|IHC-P||1/100 - 1/1000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98656 has been referenced in 1 publication.
- Xiao Q et al. Nrf3-Pla2g7 interaction plays an essential role in smooth muscle differentiation from stem cells. Arterioscler Thromb Vasc Biol 32:730-44 (2012). PubMed: 22247257