Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Biotin)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/200000. by chemiluminescent detection
ELISA 1/10000 - 1/200000. as primary
ICC/IF 1/100 - 1/500.
IHC-P 1/250 - 1/2500.

References

This product has been referenced in:

  • Alex M  et al. Astaxanthin modulates osteopontin and transforming growth factor ß1 expression levels in a rat model of nephrolithiasis: a comparison with citrate administration. BJU Int N/A:N/A (2013). Read more (PubMed: 24712822) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.
Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/concentration may vary.
Pre-adsorbed antibodies (ab5886, ab98756) are prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. This means that they only recognize mouse IgG and any cross-reactivity with other species is minimal.
I hope this helps and if I can assist further, please do not hesitate to contact me.

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