Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab98756)

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Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details

  • Minimal
    cross-reactivity


    Human, Rat more details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/200000. (Chemiluminescent).
ELISA 1/10000 - 1/200000. (Primary ELISA).
ICC/IF 1/100 - 1/500.
IHC-P 1/250 - 1/2500.

References

ab98756 has not yet been referenced specifically in any publications.

Publishing research using ab98756? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Hi,



I'm looking for a biotin labelled F(ab')2 antimouse antibody for peroxidase immunohistochemistry (lung tissue --> first antibody = monoclonal antibody IgG1 that detects adducts of hypoxyprobe). ABCAM provides three different antibodies; ab98657, ab98756 and ab5886. How do these products differ from each other? And what does pre-adsorbed means?



Many thanks in advance for your help!

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Answer

Thank you for your enquiry and your interest in our products.
Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/concentration may vary.
Pre-adsorbed antibodies (ab5886, ab98756) are prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. This means that they only recognize mouse IgG and any cross-reactivity with other species is minimal.
I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

Einen schönen Guten Morgen

Danke für Ihre Antwort und ich vermute es liegt wirklich am sec AK. Nur leider haben wir gerade keinen passenden für ab8165 da. Können Sie mir da einen empfehlen? Am besten in grün, weil rot ist schon vergeben :)

Viele Grüße

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Answer

Vielen Dank für Ihre Antwort.

Gerne empfehle ich Ihnen einen passenden Antikörper:

ab98757 (Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (DyLight® 488), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98757).

Die neuen Farbstoffe sind wesentlich Licht und pH- stabiler als konventionelle Konjugate. Bitte folgen Sie diesem Link zu einer Übersicht:

https://www.abcam.com/index.html?pageconfig=resource&rid=12934&source=pagetrap&viapagetrap=dylight

Für alle Fälle habe ich Ihnen auch einen Antikörper mit Biotin herausgesucht:

ab98756 (Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (Biotin), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98756).

Bitte lassen Sie mich wissen, ob sich Ihre Resultate verbessern. Der Antikörper ist weiterhin von unserer Abpromise Garantie abgedeckt.
Ich wünsche Ihnen viel Erfolg und verbleibe

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