Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed (ab98758)

Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Human, Rat more details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab98758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.
The user will have to verify the excitation laser and emission detection filters of their instrument.
Dylight 550 properties: Excitation at 562 nm, emission at 576 nm
PE properties: Excitation at 488 nm, emission at 578 nm
Typically, for channel 2 detection (filter = 585/40 nm) PE is a very bright fluorophore with large stokes shift (excitation with 488 laser). Dylight 550 is spectrally similar to AlexaFluor 546, AlexaFluor 555, Cy3, and TRITC.
We tend to choose PE conjugates for channel 2 detection.
I hope this helps and if we can assist further, please do not hesitate to contact us.

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Answer

It is my pleasure to help.

In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttps://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those colors.

We do offer discounts on purchases of five items or more and offer a number of promotions. Our customer service representatives will be able to give you more detailed information about our current promotions and discounts or you may follow the link here:https://www.abcam.com/index.html?pageconfig=resource&rid=14853

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for contacting us.

We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your lasers. A product such as ab96899,Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488),https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96899.html

We currently have 20 products against collagen II. However taking into account that this will have to be used in mouse tissue in conjunction with an anti-aggrecan rabbit polyclonal we can limit this to ab3092Anti-Collagen II antibody [5B2.5]. This is a mouse monoclonal antibody of isotype IgG2a that has been tested inIHC-Fr,IHC-P,WB and IF. An appropriate secondary should preferably be a F(ab) fragment as mouse on mouse staining can be difficult, please see this link for more information about mouse on mouse staining,https://www.abcam.com/index.html?pageconfig=resource&rid=11465. One such product would be ourGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed,https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fab-2-DyLight-550-pre-adsorbed-ab98758.html

Abcam also offers a number of ICC and IHC reagents. As both of the secondary antibodies listed above are raised in goat, we recommend using a 5-10% Goat serum as your blocking reagent and antibody incubation solution, ab7481https://www.abcam.com/Goat-Serum-ab7481.html. We, also offer buffers and antigen retrieval solutions,https://www.abcam.com/index.html?pageconfig=resource&rid=12854, as well as mounting media for fluorescent staining ab103748,https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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