Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Texas Red ®) preadsorbed (ab5884)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Texas Red ®) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG F(ab)2 fragment

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.7 moles Texas Red per mole of Goat IgG F(ab’)2
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Allahverdi A  et al. Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. Cell J 17:231-42 (2015). Read more (PubMed: 26199902) »
  • Jafarian A  et al. The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9. PLoS One 10:e0128650 (2015). IF . Read more (PubMed: 26047014) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

Dear Sir, 1. Please describe the problem (high background, no staining etc). No staining 2. On what material are you testing the antibody in IHC? Species? Cell line? Lymphoblast (Human) Tissue? 3. How did you fix the samples? Ethanol, methanol (Tried) Acetone (Tried) Paraformaldehyde Other (10%TCA) 4. Did you apply antigen retrieval step? Enzymatic method Heat mediated technique Other 5. How did you block the unspecific binding sites? Using 10%BSA in PBS+ 0.1% Tween 20 6. Primary antibody Specification (in which species was it raised against)? Mouse At what dilution(s) have you tested this antibody? 1:100, 1:500 and 1:1000 Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 2h. 5min washing with PBST for 15min. 7. Secondary antibody What secondary antibody are you using? anti-mouse (TR-labelled) Specification (in which species was it raised against)? goat At what dilution(s) have you tested this antibody? 1:100, 500 & 1000 Incubation, wash steps? 0.5h and 1h at R.T and 37C Do you know whether the problems you are experiencing come from the secondary? yes, since I do get signal using another aliquote of the treated cells but using another secondary Ab. What detection method are you using? Fluoresence microscopy. 8. Background staining Please provide an image of your staining I do not have a picture (As it is almost faint red with no indication about cells. However I do get very nice stainíng from DAPI. 9. Which detection system did you use? Fluoresence microscopy. 10. Did you apply positive and negative controls along with the samples? Please specify. Yes, DAPI staining to prove that I have cells on the slides. double staining the same slide with another antibody against another target and it works. 11. Optimization attempts How many times have you tried the IHC? 15 times Do you obtain the same results every time? What steps have you altered? Fixation, primary and secondary dilutions I am looking forward to hearing from you soon. Thank you for your offer. Yes I do like to try it. You can send it to the below address:

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Answer

Thank you for your e-mail and for providing more information of your protocol. I have placed an order for your -one vial of replacement vial of ab5884. For your information, the new order number is: 40004. Please do let me know how you are getting on with this replacement.

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Answer

Thank you for your quick reply and your answers to our questions. We can offer you a replacement vial - free of charge. Please do let us know if you would like to try and test it. We are looking forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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