Key features and details
- Goat F(ab')2 Anti-Mouse IgG - Fc (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Product nameGoat F(ab')2 Anti-Mouse IgG - Fc (AP), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG F(c) fragment
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 8.00
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
Our Abpromise guarantee covers the use of ab5880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/500 - 1/2500.|
|ELISA||1/2000 - 1/10000.|
|IHC||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5880 has been referenced in 1 publication.
- Maguire RJ et al. Early transcriptional targets of MyoD link myogenesis and somitogenesis. Dev Biol 371:256-68 (2012). PubMed: 22954963