Product nameGoat F(ab')2 Anti-Mouse IgG - Fc (Biotin), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG F(c) fragment
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Our Abpromise guarantee covers the use of ab5877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/20000 - 1/100000.|
|IHC||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
ab5877 has not yet been referenced specifically in any publications.