Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed (ab5879)

Submit a review Q&A (1)References (4)

Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, Human Serum Proteins.

  • Tested applications

    Suitable for: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHCmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human more details

  • Immunogen

    Mouse IgG F(c) whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/1000.
WB 1/500 - 1/2000.
Dot blot Use at an assay dependent dilution.
ELISA 1/2000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

References

This product has been referenced in:

  • Pu J  et al. Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature. Oncol Lett 12:585-590 (2016). IHC-P ; Human . Read more (PubMed: 27347184) »
  • Li H  et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). Read more (PubMed: 26446566) »
See all 4 Publications for this product

Publishing research using ab5879? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

May I ask once more? My samples are sheep proteins. I am going to use two antibodies (anti GAPDH and anti GDF8 antibodies). The first antibodies are raised in mouse and rat (mouse IgG and Rat IgG), respectively. There are many secondary antibodies (HRP conjugated) which recognize mouse IgG or rat IgG (ex. ab5870, ab5879, ab5887, ab6728.....). As some of them are raised in goat, I am afraid that I will have a high background. I would like to know which secondary antibodies against mouse and rat IgG is good for western blotting to detect sheep proteins. Thank you for your helps.

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Answer

Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I would like to recommend the following: ab6728 Mouse IgG antibody (ab6728) HRP conjugated Rabbit ab6820 Mouse IgG antibody (ab6820) HRP conjugated Donkey Both antisera have been raised in rabbit or donkey and are highly unlikely to cross react against sheep. We have also received favourable reviews for them both.

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