Product nameGoat F(ab')2 Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC, ELISA, WB, IHC-Pmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.00058% Sodium chloride, 0.0095% Magnesium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab98635 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline Phosphatase.
Our Abpromise guarantee covers the use of ab98635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|ELISA||1/1000 - 1/10000. as primary|
|WB||1/1000 - 1/10000. by chemiluminescent detection|
|IHC-P||1/100 - 1/1000.|
This product has been referenced in:
- Wagner JT & Podrabsky JE Extreme tolerance and developmental buffering of UV-C induced DNA damage in embryos of the annual killifish Austrofundulus limnaeus. J Exp Zool A Ecol Genet Physiol 323:10-30 (2015). ELISA . Read more (PubMed: 25387429) »