Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG - H&L (AP), pre-adsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG whole molecule.

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
WB 1/500 - 1/2500.
ELISA 1/9600.
IHC Use at an assay dependent dilution.

References

ab5871 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab5871.
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