Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed (ab98731)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details -
Minimal
cross-reactivity
Human, Rat more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. (Primary ELISA).
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WB |
1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminiscent: 1/10000 - 1/50000)
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|
ICC |
Use at an assay dependent dilution.
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Notes |
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IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (Primary ELISA). |
WB
1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminiscent: 1/10000 - 1/50000) |
ICC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab98731 has been referenced in 2 publications.
- Wu F et al. Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity. Acta Pharm Sin B N/A:N/A (2022). PubMed: 35846427
- Ishizaki Y et al. Development of a Caco-2 Cell Line Carrying the Human Intestine-Type CES Expression Profile as a Promising Tool for Ester-Containing Drug Permeability Studies. Biol Pharm Bull 41:697-706 (2018). PubMed: 29709907