Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed (ab6007)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
See all IgG + IgM + IgA secondary antibodies -
Description
Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. This reagent is suitable for the detection of all mouse immunoglobulin subclasses and chain combinations. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Mouse IgG, IgA and IgM whole molecules
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab6007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/500. | |
| Dot blot | Use at an assay dependent dilution. | |
| WB | 1/200 - 1/1000. | |
| ELISA | 1/6300. | |
| IHC | Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab6007 has not yet been referenced specifically in any publications.
