Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Product nameGoat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
See all IgG + IgM + IgA secondary antibodies
DescriptionGoat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. This reagent is suitable for the detection of all mouse immunoglobulin subclasses and chain combinations.
Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG, IgA and IgM whole molecules
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
Our Abpromise guarantee covers the use of ab6007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/200 - 1/1000.|
|IHC||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6007 has not yet been referenced specifically in any publications.