Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed (ab6007)

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Key features and details

  • Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC

Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed
    See all IgG + IgM + IgA secondary antibodies

  • Description

    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (AP), pre-adsorbed

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. This reagent is suitable for the detection of all mouse immunoglobulin subclasses and chain combinations.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Mouse IgG, IgA and IgM whole molecules

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/500.
Dot blot
Use at an assay dependent dilution.
WB
1/200 - 1/1000.
ELISA
1/6300.
IHC
Use at an assay dependent dilution.
Notes
ICC/IF
1/100 - 1/500.
Dot blot
Use at an assay dependent dilution.
WB
1/200 - 1/1000.
ELISA
1/6300.
IHC
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab6007? Please let us know so that we can cite the reference in this datasheet.

ab6007 has not yet been referenced specifically in any publications.

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