Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (TRITC) preadsorbed (ab6004)

Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (TRITC) preadsorbed
    See all IgG + IgM + IgA secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Human more details
  • Immunogen

    Mouse IgG, IgA, and IgM whole molecules

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 3.5 moles TRITC per mole of Goat IgG
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

References

ab6004 has not yet been referenced specifically in any publications.

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