Key features and details
- Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, WB, ELISA
Product nameGoat F(ab')2 Anti-Mouse IgM mu chain
See all IgM secondary antibodies
DescriptionGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Mouse IgM mu heavy chain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.878% Sodium chloride
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Our Abpromise guarantee covers the use of ab5925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|WB||1/200 - 1/2000.|
|ELISA||1/2000 - 1/8000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5925 has not yet been referenced specifically in any publications.