Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

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Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

    Key features and details

    • Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    • Conjugation: Biotin
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cyt

    Overview

    • Product name

      Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
      See all IgM secondary antibodies

    • Host species

      Goat

    • Target species

      Mouse

    • Specificity

      Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

    • Tested applications

      Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details

    • Immunogen

      Mouse IgM mu heavy chain

    • Conjugation

      Biotin

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
    • Concentration information loading...

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    • Conjugation notes

      Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5929 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/50000.
    WB
    1/1000 - 1/5000.
    Dot blot
    Use at an assay dependent dilution.
    IHC
    1/500 - 1/5000.
    Immunomicroscopy
    Use at an assay dependent dilution.
    Flow Cyt
    1/200 - 1/1000.
    Notes
    ICC/IF
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/50000.
    WB
    1/1000 - 1/5000.
    Dot blot
    Use at an assay dependent dilution.
    IHC
    1/500 - 1/5000.
    Immunomicroscopy
    Use at an assay dependent dilution.
    Flow Cyt
    1/200 - 1/1000.

    Images

    • Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
      Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

      ab5929 was used as the secondary antibody in WB.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab5929? Please let us know so that we can cite the reference in this datasheet.

    ab5929 has been referenced in 2 publications.

    • Hong R  et al. Distinct roles of BCNP1 in B-cell development and activation. Int Immunol 32:17-26 (2020). PubMed: 31412363
    • Grewal N  et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . PubMed: 25502628

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question


    I have some questions for you. Recently, I tried to use your antibodies, ab49394(primary AB) and ab5929( 2nd antibody), doing IHC for measure macrophages of small intestine tissue of human for a few times. I got good stains for positive samples without background, but negative control(without primary antibody)also got some positive spots. I tried to block it, I used:
    1. 5% BSA in TBS with tween 20, 2 hours at RT;
    2. 5% normal goat serum in TBS, 2 hours at RT;
    3. I also blocked endogenous Avidin used vector Lab,SP-2002 and endogenous HRP used Dako, S2003.
    Could you give me some suggestions to solute this problem?

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    Answer

    Thank you for contacting Abcam.

    I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positive and negative sections. By being able to see the staining that you are getting, I will hopefully be able to resolve this issue quickly for you.

    I look forward to your reply.

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    Question

    I am working with B cells which express an human IgG or human IgM as well as a murine IgM. I would like to show the expression of the murine IgM in FACS. Therefore I need an antibody which ONLY recognizes the murine IgM but not the human IgM or IgG. In your catalogue I found an "Mouse IgM secondary antibody - µ chain speciic- F(ab)2 Fragment (ab5932). Do you know if this antibody crossreacts with human IgM/G? Unfortunately this antibody is coupled to PE. Since my cells are GFP positive I prefere Biotin or APC labeled antibodies. Do you have an anti mouse-µ antibody not reacting with human IgG/M which is coupled to Biotin or APC?

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    Answer

    We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cross adsorbed or tested against human IgM, and we cannot guarantee their suitable for your assay. Two other biotin conjugated anti-mouse IgG antibodies but only tested in ELISA: ab11659 Mouse IgM secondary antibody [LO-MM-9] - Heavy Chain specific. Unable to guarantee non-reactivity with human Ig. ab49760 will not recognise other mouse Ig's as there was no reaction against IgA, IgG, and IgM. Human IgG or IgM cross-reactivity has not been tested.

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