Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    See all IgM secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Mouse IgM mu heavy chain

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
IHC 1/500 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/200 - 1/1000.

Images

  • Ab5929 was used with the primary antibody ab8226 in WB. See Abreview on ab8226.

References

This product has been referenced in:
  • Grewal N  et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . Read more (PubMed: 25502628) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positive and negative sections. By being able to see the staining that you are getting, I will hopefully be able to resolve this issue quickly for you.

I look forward to your reply.

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Answer

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cross adsorbed or tested against human IgM, and we cannot guarantee their suitable for your assay. Two other biotin conjugated anti-mouse IgG antibodies but only tested in ELISA: ab11659 Mouse IgM secondary antibody [LO-MM-9] - Heavy Chain specific. Unable to guarantee non-reactivity with human Ig. ab49760 will not recognise other mouse Ig's as there was no reaction against IgA, IgG, and IgM. Human IgG or IgM cross-reactivity has not been tested.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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