Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)

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Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
    See all IgM secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

  • Tested applications

    Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details

  • Immunogen

    Mouse IgM mu heavy chain

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.
WB 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
IHC 1/500 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/200 - 1/1000.

Images

  • Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
    Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
    Ab5929 was used with the primary antibody ab8226 in WB. See Abreview on ab8226.

References

This product has been referenced in:

  • Grewal N  et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . Read more (PubMed: 25502628) »
See 1 Publication for this product

Publishing research using ab5929? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question


I have some questions for you. Recently, I tried to use your antibodies, ab49394(primary AB) and ab5929( 2nd antibody), doing IHC for measure macrophages of small intestine tissue of human for a few times. I got good stains for positive samples without background, but negative control(without primary antibody)also got some positive spots. I tried to block it, I used:
1. 5% BSA in TBS with tween 20, 2 hours at RT;
2. 5% normal goat serum in TBS, 2 hours at RT;
3. I also blocked endogenous Avidin used vector Lab,SP-2002 and endogenous HRP used Dako, S2003.
Could you give me some suggestions to solute this problem?

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Answer

Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with one of our antibodies. To be able to help further in this matter, would you be able to supply some images of the staining that you are with both your positive and negative sections. By being able to see the staining that you are getting, I will hopefully be able to resolve this issue quickly for you.

I look forward to your reply.

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Question

I am working with B cells which express an human IgG or human IgM as well as a murine IgM. I would like to show the expression of the murine IgM in FACS. Therefore I need an antibody which ONLY recognizes the murine IgM but not the human IgM or IgG. In your catalogue I found an "Mouse IgM secondary antibody - µ chain speciic- F(ab)2 Fragment (ab5932). Do you know if this antibody crossreacts with human IgM/G? Unfortunately this antibody is coupled to PE. Since my cells are GFP positive I prefere Biotin or APC labeled antibodies. Do you have an anti mouse-µ antibody not reacting with human IgG/M which is coupled to Biotin or APC?

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Answer

We do not have any APC labeled IgM antibodies. Other conjugates we have- Texas red, AP, HRP,FITC, Rhodamine. ab5929- Biotin labelled. Seems to be the best option as it is also tested in Flow cytometry. However, neither ab5929 or ab5932 is cross adsorbed or tested against human IgM, and we cannot guarantee their suitable for your assay. Two other biotin conjugated anti-mouse IgG antibodies but only tested in ELISA: ab11659 Mouse IgM secondary antibody [LO-MM-9] - Heavy Chain specific. Unable to guarantee non-reactivity with human Ig. ab49760 will not recognise other mouse Ig's as there was no reaction against IgA, IgG, and IgM. Human IgG or IgM cross-reactivity has not been tested.

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