Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cyt
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). -
Tested applications
Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details -
Immunogen
Mouse IgM mu heavy chain
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/2500.
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ELISA |
1/10000 - 1/50000.
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WB |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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IHC |
1/500 - 1/5000.
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Immunomicroscopy |
Use at an assay dependent dilution.
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Flow Cyt |
1/200 - 1/1000.
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Notes |
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ICC/IF
1/500 - 1/2500. |
ELISA
1/10000 - 1/50000. |
WB
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
IHC
1/500 - 1/5000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/200 - 1/1000. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab5929 has been referenced in 1 publication.
- Grewal N et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . PubMed: 25502628