Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)

Submit an Abreview Q&A (1)Specific References (6)

Overview

  • Product name
    Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
    See all IgM secondary antibodies
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Immunogen

    Mouse IgM mu chain

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.3% Potassium phosphate
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
Dot blot 1/5000 - 1/100000.
WB 1/2000 - 1/10000.
ELISA 1/90000.
Electron Microscopy Use at an assay dependent dilution.
IHC 1/500 - 1/5000.

References

This product has been referenced in:
  • Hasham MG  et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). ELISA . Read more (PubMed: 28250051) »
  • Rieder LE  et al. Dynamic response of RNA editing to temperature in Drosophila. BMC Biol 13:1 (2015). Read more (PubMed: 25555396) »

See all 6 Publications for this product

Publishing research using ab5930? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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I finished western blotting experiment yesterday. The expression of CYP1A1 and telomerase in lung cancer cell lines were detected. There is no signal developed on the telomerase membrane. Multi-bands were developed on CYP1A1 memebrane (Please find ...

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Thank you for your enquiry. Well done on getting your western blot to work. I have read your comments and have examined your blot images. I would like ot take each of your antibodies one at a time: ab3568 - Cytochrome P450 1A1 antibody (ab3...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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