Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHC
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
Tested applications
Suitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Immunogen
Mouse IgM mu chain
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.87% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/5000.
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IHC-Fr |
Use at an assay dependent dilution.
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|
Dot blot |
1/5000 - 1/100000.
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WB |
1/2000 - 1/10000.
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ELISA |
1/90000.
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Electron Microscopy |
Use at an assay dependent dilution.
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|
IHC |
1/500 - 1/5000.
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Notes |
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ICC/IF
1/500 - 1/5000. |
IHC-Fr
Use at an assay dependent dilution. |
Dot blot
1/5000 - 1/100000. |
WB
1/2000 - 1/10000. |
ELISA
1/90000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
1/500 - 1/5000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab5930 has been referenced in 10 publications.
- Hou X et al. Wnt signaling regulates cytosolic translocation of connexin 43. Am J Physiol Regul Integr Comp Physiol 317:R248-R261 (2019). PubMed: 31067079
- Lynn MA et al. Early-Life Antibiotic-Driven Dysbiosis Leads to Dysregulated Vaccine Immune Responses in Mice. Cell Host Microbe 23:653-660.e5 (2018). PubMed: 29746836
- Xu W et al. Emission of sulfur dioxide from polyurethane foam and respiratory health effects. Environ Pollut 242:90-97 (2018). PubMed: 29960929
- Sahin A et al. Human SOD1 ALS Mutations in a Drosophila Knock-In Model Cause Severe Phenotypes and Reveal Dosage-Sensitive Gain- and Loss-of-Function Components. Genetics 205:707-723 (2017). PubMed: 27974499
- Hasham MG et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). ELISA . PubMed: 28250051
- Rieder LE et al. Dynamic response of RNA editing to temperature in Drosophila. BMC Biol 13:1 (2015). PubMed: 25555396
- Kuge H et al. Functional compartmentalization of the plasma membrane of neurons by a unique acyl chain composition of phospholipids. J Biol Chem 289:26783-93 (2014). ELISA . PubMed: 25096572
- Oksenberg N et al. Function and regulation of AUTS2, a gene implicated in autism and human evolution. PLoS Genet 9:e1003221 (2013). PubMed: 23349641
- Orihuela CJ et al. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. J Clin Invest : (2009). IHC-Fr ; Mouse . PubMed: 19436113
- Shanley DK & Sullivan AM Expression of the cell surface markers mAb 2F7 and PSA-NCAM in the embryonic rat brain. Neurosci Lett 424:165-9 (2007). WB ; Mouse . PubMed: 17723277