Goat F(ab')2 Anti-Mouse IgM mu chain (PE) (ab5932)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (PE)
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgM mu chain (PE)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or human, bovine or horse serum proteins. -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cytmore details -
Immunogen
Mouse IgM mu heavy chain
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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Immunomicroscopy |
Use at an assay dependent dilution.
As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications. |
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Flow Cyt |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
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Notes |
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ICC/IF
1/100 - 1/250. |
Immunomicroscopy
Use at an assay dependent dilution. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications. |
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab5932 has been referenced in 3 publications.
- Sahu G et al. Junctophilin Proteins Tether a Cav1-RyR2-KCa3.1 Tripartite Complex to Regulate Neuronal Excitability. Cell Rep 28:2427-2442.e6 (2019). PubMed: 31461656
- Chen J & Liu F Analysis of ABO chimera from peripheral red cells and reticulocytes by flow cytometry and micro gel column technique in patients post-ABO-incompatible HSCT. Clin Transplant 32:N/A (2018). PubMed: 29226393
- Lappalainen J et al. A protocol for generating high numbers of mature and functional human mast cells from peripheral blood. Clin Exp Allergy 37:1404-14 (2007). Flow Cyt ; Mouse . PubMed: 17845422