Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio: 2.4 moles Texas Red per mole of Goat IgG F(ab’)2