Overview

  • Product name

    Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC)
    See all IgM secondary antibodies
  • Description

    Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (TRITC )
  • Host species

    Goat
  • Target species

    Mouse
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
  • Tested applications

    Suitable for: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Mouse IgM mu chain

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG F(ab’)2
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.

References

This product has been referenced in:

  • Ishikawa M  et al. Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci 55:8531-41 (2014). ICC/IF ; Rat . Read more (PubMed: 25406290) »
See 1 Publication for this product

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