Goat F(ab')2 Anti-Rabbit IgG - (Fab')2 (Biotin), pre-adsorbed (ab102290)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - (Fab')2 (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Rabbit IgG - (Fab')2 (Biotin), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-P, ICC, ELISA, WBmore details -
Minimal
cross-reactivity
Human, Mouse, Rat more details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies.The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500 - 1/5000.
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ICC |
1/200 - 1/1000.
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ELISA |
1/10000 - 1/200000. (Primary).
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WB |
1/10000 - 1/200000. (Chemiluminescent).
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Notes |
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IHC-P
1/500 - 1/5000. |
ICC
1/200 - 1/1000. |
ELISA
1/10000 - 1/200000. (Primary). |
WB
1/10000 - 1/200000. (Chemiluminescent). |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab102290 has not yet been referenced specifically in any publications.