Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)


  • Product name
    Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins..
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Minimal

    Cow, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG F(ab’)2 fragment

  • Conjugation


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab6112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/100000.

This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Electron Microscopy Use at an assay dependent concentration.
IHC Use at an assay dependent dilution.


  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6112 was used at dilution 1/5000 with the primary antibody ab32117 in WB. See the review on ab32117.


This product has been referenced in:
  • Fallah H  et al. Berberis integerrima ameliorates insulin resistance in high- fructose-fed insulin-resistant rats. Iran J Basic Med Sci 20:1093-1101 (2017). Read more (PubMed: 29147484) »
  • Mohammadi A  et al. Antihyperglycemic Effect ofRosa Damascenais Mediated by PPAR.? Gene Expression in Animal Model of Insulin Resistance. Iran J Pharm Res 16:1080-1088 (2017). Read more (PubMed: 29201096) »
See all 12 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:






It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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Thank you for your enquiry. I apologize as there was a mistake on the datasheet for ab6112 - the antibody storage buffer contains 0.01% Gentamicin sulfate and not sodium azide. I have updated the datasheet to reflect this. The immunogen used to generate ab6112 was Rabbit IgG F(ab')2 fragment. The immunogen used to generate ab6013 was Rabbit IgG, whole molecule. This is the difference between these two antibodies. All of our secondary antibodies are priced at $150.00. It is up to you to decide which antibody better suits your needs. If you have any additional questions, please contact us again.

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