Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)

Reviews (1)Q&A (1)References (5)

Key features and details

  • Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold)
  • Conjugation: Gold 10nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Electron Microscopy

Overview

  • Product name

    Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Tested applications

    Suitable for: Electron Microscopymore details

  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Notes
Application notes
Electron Microscopy: Use at an assay dependent dilution.
Light Microscopy: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab39601? Please let us know so that we can cite the reference in this datasheet.

ab39601 has been referenced in 5 publications.

  • Wong JM  et al. Postsynaptic Serine Racemase Regulates NMDA Receptor Function. J Neurosci 40:9564-9575 (2020). PubMed: 33158959
  • Chen T  et al. Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda). J Morphol 280:1170-1184 (2019). PubMed: 31141207
  • Zhang ZH  et al. Dynamics of histone H2A, H4 and HS1ph during spermatogenesis with a focus on chromatin condensation and maturity of spermatozoa. Sci Rep 6:25089 (2016). PubMed: 27121047
  • Occhialini A  et al. Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2. Plant J 85:148-60 (2016). PubMed: 26662726
  • Wu JL  et al. Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. PLoS One 10:e0126623 (2015). PubMed: 25993499

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1-2 of 2 Abreviews or Q&A

immunoelctron microscopic localisation of a KAP protein in human hair follicles

 Excellent
Abreviews
Application
Immunohistochemistry
Human skin containing 3-4 hair follicles was fixed in glutaraldehyde/paraformaldehyde, embedded in London resin, sectioned at 75nm, subjected to a protocol of potassium periodate antigen retrieval and treated with 2ug/ml of a rabbit anti- KRTAP5 antibody followed by Abcam Goat anti-rabbit IgG 10nm gold. at a dilution of 1/25 The localisation was excellent and specific to the keratin in the cuticle and cortex layers of the developing cuticle and cortex. The binding is to the exocuticle and also the darker layer at the edges of the cuticle cells, the a-layer Reaction also occurs with the cortex (upper right) because the KAPs in the cortex share amino acid sequences. Control (no antibody) was negative.
GE Rogers
Biochemistry
The University of Adelaide' South Australia

Abcam user community

Verified customer

Submitted Dec 08 2014

Question

What is the concentration?

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Answer

The F(ab')2 concentration for ab39601, Goat F(ab')2 Anti-Rabbit IgG 10nm gold conjugate, is 10 microgram/ml. The gold particle concentration of the 10nm gold conjugate is approximately 5x10e12 particles per ml.

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