Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold) (ab39601)

Reviews (1)Q&A (1)References (3)

Overview

  • Product name

    Goat F(ab')2 Anti-Rabbit IgG H&L (10nm Gold)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Tested applications

    Suitable for: Electron Microscopymore details

  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.
    Light Microscopy: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    This product has been referenced in:

    • Zhang ZH  et al. Dynamics of histone H2A, H4 and HS1ph during spermatogenesis with a focus on chromatin condensation and maturity of spermatozoa. Sci Rep 6:25089 (2016). Read more (PubMed: 27121047) »
    • Occhialini A  et al. Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2. Plant J 85:148-60 (2016). Read more (PubMed: 26662726) »
    See all 3 Publications for this product

    Publishing research using ab39601? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    What is the concentration?

    Read More
    Answer

    The F(ab')2 concentration for ab39601, Goat F(ab')2 Anti-Rabbit IgG 10nm gold conjugate, is 10 microgram/ml. The gold particle concentration of the 10nm gold conjugate is approximately 5x10e12 particles per ml.

    Read More

    immunoelctron microscopic localisation of a KAP protein in human hair follicles

     Excellent
    Abreviews
    Application
    Immunohistochemistry
    Human skin containing 3-4 hair follicles was fixed in glutaraldehyde/paraformaldehyde, embedded in London resin, sectioned at 75nm, subjected to a protocol of potassium periodate antigen retrieval and treated with 2ug/ml of a rabbit anti- KRTAP5 antibody followed by Abcam Goat anti-rabbit IgG 10nm gold. at a dilution of 1/25 The localisation was excellent and specific to the keratin in the cuticle and cortex layers of the developing cuticle and cortex. The binding is to the exocuticle and also the darker layer at the edges of the cuticle cells, the a-layer Reaction also occurs with the cortex (upper right) because the KAPs in the cortex share amino acid sequences. Control (no antibody) was negative.
    GE Rogers
    Biochemistry
    The University of Adelaide' South Australia

    Abcam user community

    Verified customer

    Submitted Dec 08 2014

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