This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline Phosphatase.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/100 - 1/1000.
1/1000 - 1/10000. (Primary ELISA)
1/1000 - 1/10000. (Chemiluminescent)
This product has been referenced in:
Li Y et al. Oral immunization with rotavirus VP7-CTB fusion expressed in transgenic Arabidopsis thaliana induces antigen-specific IgA and IgG and passive protection in mice. Exp Ther Med15:4866-4874 (2018).
Read more (PubMed: 29805507) »