Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed (ab6012)

Submit a review Q&A (1)References (5)

Key features and details

  • Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopy

Overview

  • Product name

    Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6012 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent dilution. PubMed: 21498392
    Dot blot
    Use at an assay dependent dilution.
    WB
    1/2000 - 1/10000.
    ELISA
    1/20000 - 1/100000.
    IHC
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    Notes
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent dilution. PubMed: 21498392
    Dot blot
    Use at an assay dependent dilution.
    WB
    1/2000 - 1/10000.
    ELISA
    1/20000 - 1/100000.
    IHC
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (5)

    Publishing research using ab6012? Please let us know so that we can cite the reference in this datasheet.

    ab6012 has been referenced in 5 publications.

    • Webb TE  et al. An internal ribosome entry site in the 5' untranslated region of epidermal growth factor receptor allows hypoxic expression. Oncogenesis 4:e134 (2015). WB ; Human . PubMed: 25622307
    • Huang R  et al. Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma. Oncol Lett 10:2812-2820 (2015). IHC . PubMed: 26722247
    • Wang Z  et al. Changes in large pulmonary arterial viscoelasticity in chronic pulmonary hypertension. PLoS One 8:e78569 (2013). IHC (PFA fixed) . PubMed: 24223157
    • Kacevska M  et al. Extrahepatic cancer suppresses nuclear receptor-regulated drug metabolism. Clin Cancer Res 17:3170-80 (2011). IHC-P . PubMed: 21498392
    • Chamberlain G  et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt . PubMed: 18698345

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
    Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
    Customer concern cross reaction of secondary anibody.
    Which secondary antibody you suggest since their brain sample also from mouse.
    Thanks a lot for your assistance.
    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

    We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

    https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

    https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

    https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

    https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

    https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

    It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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