Product nameGoat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Tested applicationsSuitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rabbit IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Our Abpromise guarantee covers the use of ab6012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent dilution. PubMed: 21498392|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/20000 - 1/100000.|
|IHC||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
This product has been referenced in:
- Webb TE et al. An internal ribosome entry site in the 5' untranslated region of epidermal growth factor receptor allows hypoxic expression. Oncogenesis 4:e134 (2015). WB ; Human . Read more (PubMed: 25622307) »
- Huang R et al. Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma. Oncol Lett 10:2812-2820 (2015). IHC . Read more (PubMed: 26722247) »