Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab6013)

Overview

  • Product name
    Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Rabbit
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/115000.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.

References

This product has been referenced in:
  • Westcott NP  et al. Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. Nat Chem Biol 13:302-308 (2017). Read more (PubMed: 28092360) »
  • Chou MH  et al. Effects of hepatocyte CD14 upregulation during cholestasis on endotoxin sensitivity. PLoS One 7:e34903 (2012). WB . Read more (PubMed: 22511970) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
I used 1:1000 dilution of this antibody to detect anti-SOX9 primary antibody.

Abcam user community

Verified customer

Submitted Jun 04 2018

Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

Read More

Answer

Thank you for your enquiry. I apologize as there was a mistake on the datasheet for ab6112 - the antibody storage buffer contains 0.01% Gentamicin sulfate and not sodium azide. I have updated the datasheet to reflect this. The immunogen used to generate ab6112 was Rabbit IgG F(ab')2 fragment. The immunogen used to generate ab6013 was Rabbit IgG, whole molecule. This is the difference between these two antibodies. All of our secondary antibodies are priced at $150.00. It is up to you to decide which antibody better suits your needs. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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