Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab98512)

Overview

  • Product name

    Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Description

    Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (as Primary).
WB 1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.

References

ab98512 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

The Goat anti-rabbit HPR Conjugated used in AB80436 has been developed specifically for use in this kit and is currently not available for separate purchase. We do offer a number of Goat anti-rabbit secondaries which are HRP conjugated and have been validated for used in IHC. AB7090 and AB98512 would each be excellent choices for any IHC against an antibody raised in rabbit. Each are pre-absorbed to reduce cross reactivity, and AB98512 is an F(ab')2 fragment. Use of F(ab')2 fragments further reduce non-specific background as these antibodies lack the Fc region which may bind endogenous Fc receptors within the tissue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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