Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IHC, ICC/IF, Dot blot, WB
Product nameGoat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG F(ab)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat Fc or Bovine, Horse, and Human Serum Proteins
Tested applicationsSuitable for: ELISA, IHC, ICC/IF, Dot blot, WBmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rat IgG F(ab)2 fragment
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 1.5% BSA, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/50000.|
|IHC||Use at an assay dependent dilution.|
|ICC/IF||1/200 - 1/1000.|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/5000 - 1/50000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6518 has not yet been referenced specifically in any publications.