Goat F(ab')2 Anti-Rat IgG - (Fab')2 (FITC), pre-adsorbed (ab6266)

Submit a review Submit a question References (2)

Key features and details

  • Goat F(ab')2 Anti-Rat IgG - (Fab')2 (FITC), pre-adsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA

Overview

  • Product name

    Goat F(ab')2 Anti-Rat IgG - (Fab')2 (FITC), pre-adsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rat

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Pepsin, anti-Goat IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details

  • Minimal
    cross-reactivity


    Cow, Horse, Human more details

  • Immunogen

    Rat IgG F(ab’)2 fragment

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • Conjugation notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.1 moles FITC per mole of Goat IgG F(ab’)2

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6266 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Flow Cyt
    Immunomicroscopy
    ELISA
    Notes
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab6266? Please let us know so that we can cite the reference in this datasheet.

    ab6266 has been referenced in 2 publications.

    • Ufimtseva E Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro. J Immunol Res 2016:4325646 (2016). PubMed: 27066505
    • Ufimtseva E Mycobacterium-Host Cell Relationships in Granulomatous Lesions in a Mouse Model of Latent Tuberculous Infection. Biomed Res Int 2015:948131 (2015). PubMed: 26064970

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab6266.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com