Product nameGoat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
See all IgG secondary antibodies
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rat IgG F(ab’)2 fragment
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.16% Sodium phosphate
BSA is IgG and protease free
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab6517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
|ELISA||1/10000 - 1/100000.|
|Electron Microscopy||Use at an assay dependent dilution.|
|IHC||Use at an assay dependent dilution.|
This product has been referenced in:
- Motomura E et al. Cell-autonomous signal transduction in the Xenopus egg Wnt/ß-catenin pathway. Dev Growth Differ 56:640-52 (2014). WB . Read more (PubMed: 25330272) »
- Wang SH et al. Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. Glycobiology N/A:N/A (2013). Read more (PubMed: 23363740) »